Practical skills · Fieldwork · SL & HL

Quadrat sampling explorer

Learn how quadrats sample non-mobile organisms, then carry out a random quadrat sample yourself.

When could this come up?

This is an important skill for the IA, especially if you want to investigate how plants or other non-mobile organisms change across different locations.

In exams, you may also be asked to comment on the abundance of a species and what this could mean for an ecosystem, describe a method for measuring population abundance, choose an appropriate sampling technique, or interpret quadrat data.

You therefore need to understand both how the sample is collected and how the data are calculated and interpreted.

How it works

A quadrat is a frame of a known size placed over part of a habitat. It may be divided into smaller grid squares to make measurements such as percentage cover and percentage frequency easier.

1

Decide what is being measured: abundance, population density, percentage frequency or percentage cover.

2

Choose a quadrat size suitable for the organisms.

3

Choose a sampling method appropriate to the investigation.

4

Take enough quadrat samples to make the results representative.

Choosing quadrat size

10 × 10 cm0.01 m²Very small organisms, e.g. lichens or algae
0.5 × 0.5 m0.25 m²Grasses, herbs, small shrubs, mussels or limpets
1 × 1 m1 m²Medium-sized plants and larger herbs
5 × 5 m25 m²Larger vegetation such as mature trees
How many samples? More quadrats generally improve reliability, but take more time. Increase sampling effort until additional quadrats cause little change in the overall result.

Choose the sampling technique

Click each method to see how the sample positions are chosen and when the method is useful.

Calculating the results

Mean abundance

Add all counts and divide by the number of quadrats.

total count ÷ number of quadrats
Population density

Divide abundance by the area sampled.

abundance ÷ area
Percentage frequency

The share of sampling units that contain the species.

units with the species ÷ total units × 100
Percentage cover

Estimate the proportion of quadrat area covered.

covered area ÷ quadrat area × 100

Worked example: clover

This 1 m² quadrat is divided into 25 equal squares. Seven individual clover plants occur across six occupied grid squares.

Example 1 m × 1 m quadrat

AbundanceCount individuals.
7 clover plants
Population densityAbundance ÷ area.
7 ÷ 1 m² = 7 plants m⁻²
Percentage frequencyOccupied squares ÷ total squares × 100.
6 ÷ 25 × 100 = 24%
Percentage coverEstimate the actual area covered by the plant.
Area estimate, not a count
Frequency can also use separate quadrats. If clover occurs in 3 of 5 sampled quadrats, frequency across those quadrats is 60%.
1. MapGenerate a random coordinate
2. PlaceDrag the quadrat to the grid
3. MeasureEstimate before revealing
4. RepeatCompare your samples

1. Random quadrat sampling

This is random sampling. The study area is divided into 30 numbered squares. A random number is generated to decide which square must be sampled, so the student does not choose the location themselves. This helps reduce selection bias.

Random sampling method: 1. Divide the study area into numbered squares. 2. Generate a random number. 3. Place the quadrat in that numbered square. 4. Record the organisms inside. 5. Repeat using new random numbers.
Random square number
Because the number is selected by chance, this is random sampling.
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Your quadrat
Drag this onto the numbered square selected at random.

Sampling record

No quadrats recorded yet.

Storage size and resilience

A disease affects the daisy population in this meadow. Explain why a larger population of daisies, representing a larger biological storage within the system, could make the ecosystem more resilient to this disturbance.

IB ESS revision on FirstHandIB